Second Harmonic Generation Imaging

Second-Harmonic Generation (SHG) microscopy is a cutting-edge, label-free imaging technique that enables high-resolution visualization of live cells and tissues. Unlike conventional fluorescence microscopy, SHG relies on nonlinear optical properties of non-centrosymmetric molecules, providing unique contrast without the need for exogenous dyes. This makes it particularly valuable for studying structural proteins, such as collagen and microtubules, and for applications in tissue engineering, disease modeling, and biomedical research.

Key Features

• Comprehensive coverage from basic physics to practical implementation, including step-by-step guidance on building an SHG microscope.
• Comparison with related imaging modalities to clarify strengths and limitations of SHG.
• Detailed discussion of imaging methods and analysis for tissues and live cells, covering cell membranes, muscle fibers, collagen networks, and cytoskeletal components.
• Insightful overview of biomedical applications, including cancer detection, fibrotic and autoimmune diseases, connective tissue disorders, eye pathologies, and cardiovascular disease.
• Emphasis on quantitative and objective metrics to complement traditional histology, enhancing accuracy and reproducibility in research and clinical studies.
• Contributions from leading experts and pioneers, providing both a tutorial for newcomers and a state-of-the-art review for experienced researchers.

Product Details

• Publisher: CRC Press .1 edition (June 18 2013)
• Language: English
• ISBN-10 ‏ : ‎ 1439849145
• ISBN-13 ‏ : ‎ 978-1439849149